Protocols
Fab labeling protocol for antigen microarrays (Word Forma; updated 6/25/2008)
Antibody Sources (Excel)
Lysis Buffer Recipe (Word Format)
Reverse Phase Microarray Protocol (Word Format)
Lists of antigens can be found in the following references:
Sekine H., K. L. Graham, S. Zhao, M.K. Elliott, P. Ruiz, L. Morel, V. M. Holers, P.J. Utz and G. S. Gilkeson. Role of MHC-linked genes in autoantigen selection and renal disease in a murine model of SLE. J. Immunol. 2006. In press.
Kattah M.G., G.R. Alemi, D.L. Thibault and P.J. Utz. A novel two-color fab labeling method for autoantigen protein microarrays. Nature Methods. 2006. In press.
Graham K.L., D. L. Thibault, M. Kamachi, S. Zhao, J. Steinman, L. Okeke, P. N. Kao and P.J. Utz. Granzyme B is dispensable for immunological tolerance to self in a murine model of systemic lupus erythematosus. Arth. Rheum. 2005. 52:1684-1693.
Graham, K.L., K.E. Gustin, C. Rivera, N.M. Kuyumcu-Martinez, S.S. Choe, R.E. Lloyd P. Sarnow and P.J. Utz. Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection. J. Virol 2004. 78:6313-21.
Robinson, W.H., Carla DiGennaro, Wolfgang Hueber, Brian B. Haab, Makoto Kamachi, Erik J. Dean, Sylvie Fournel, Derek Fong, Mark C. Genovese, Henry E. Neuman de Vegvar, Günter Steiner, David L. Hirschberg, Robert I. Morris, Sylviane Muller, Ger J. Pruijn, Walther J. van Venrooij, Josef S. Smolen, Patrick O. Brown, Lawrence Steinman and P.J. Utz. Autoantigen Microarrays for Multiplex Characterization of Autoantibody Responses. Nature Medicine. 2002. 8:295-301.
and link them to the Sekine (2006), Kattah (2006), Graham (2004 and 2006) and Robinson paper (2002)
